The Minimum Inhibitory Concentration (MIC) of the antimicrobial in use might be affected either in a synergistic fashion or antagonistic manner by other chemicals in the liquid being tested. Therefore it is always recommended to conduct a calibration for the antimicrobial being used in a sample of the liquid.
Standardise your test protocol as much as possible:
- Standardise the time of removal from refrigerated storage before use. Ensure adequate time is allowed for ampules to reach ambient temperature.
- Standardise the incubation period for each ampule at 3 hours and 15 minutes (or a time you have decided on for your testing).
- Ensure each operator understands what is a positive and negative result and thus avoid subjective operator errors.